EOMA 小鼠血管內(nèi)皮瘤細(xì)胞

產(chǎn)品型號：CRL-2586
廠商性質(zhì)：生產(chǎn)廠家
更新時間：2024-11-14
訪問量：2092

產(chǎn)品咨詢聯(lián)系我們

產(chǎn)品分類

Product Category

細(xì)胞庫

細(xì)胞原代牛、狗、雞、鴨、魚原代細(xì)胞羊原代細(xì)胞豬原代細(xì)胞兔原代細(xì)胞小鼠原代細(xì)胞大鼠原代細(xì)胞人原代細(xì)胞培養(yǎng)基原代細(xì)胞凍存液 ATCC 人乳腺癌豬正常細(xì)胞豬睪丸細(xì)胞系人膠質(zhì)瘤細(xì)胞人成骨細(xì)胞人永生化肝細(xì)胞人肝癌細(xì)胞人卵巢癌細(xì)胞人結(jié)直腸腺癌人經(jīng)典小細(xì)胞肺癌細(xì)胞人小細(xì)胞肺癌細(xì)胞人結(jié)直腸腺癌細(xì)胞人鼻腔上皮細(xì)胞中國倉鼠卵巢懸浮細(xì)胞嗜性逆轉(zhuǎn)錄病毒包裝穩(wěn)定細(xì)胞株小鼠神經(jīng)細(xì)胞瘤人眼脈洛膜黑色素瘤 PT67 逆轉(zhuǎn)錄酶病毒包裝細(xì)胞鴨子胚胎成纖維細(xì)胞豬腎細(xì)胞系昆蟲細(xì)胞貓腎細(xì)胞恒河猴胚腎細(xì)胞倉鼠卵巢細(xì)胞負(fù)鼠腎細(xì)胞倉鼠肺細(xì)胞果蠅胚胎細(xì)胞家貓肺成纖維樣細(xì)胞雞胚細(xì)胞豚鼠心肌來源細(xì)胞豚鼠肺成纖維樣細(xì)胞豚鼠皮膚成纖維樣細(xì)胞狗腎細(xì)胞非洲綠猴胚胎腎上皮細(xì)胞猴胚胎腎上皮細(xì)胞長臂猿淋巴瘤新生牛眼囊成纖維細(xì)胞黑化大家鼠肺成纖維樣細(xì)胞俄勒岡地鼠細(xì)胞家兔皮膚成纖維樣細(xì)胞昆蟲卵巢細(xì)胞人胸膜瘤細(xì)胞非洲綠猴腎細(xì)胞 VERO 衍生株（SVP）白化金黃地鼠肺成纖維樣細(xì)胞黃胸鼠肺成纖維樣細(xì)胞白鰱尾鰭細(xì)胞蝙蝠肺細(xì)胞草魚腎細(xì)胞系大黃魚腎細(xì)胞猴腎細(xì)胞系犬腎細(xì)胞系人結(jié)腸直腸腺癌豬肺泡巨噬細(xì)胞 PFHSA05（TLL2）人重組特洛德樣蛋白因子 PFHSA04 （BMP1）人重組骨誘導(dǎo)因子人乳腺癌細(xì)胞結(jié)腸癌細(xì)胞成纖維細(xì)胞人胚腎細(xì)胞動物卵巢細(xì)胞動物胚胎細(xì)胞動物肺細(xì)胞肋骨來源細(xì)胞成纖維樣細(xì)胞動物上皮細(xì)胞動物肝臟細(xì)胞可誘導(dǎo)表達(dá)穩(wěn)定細(xì)胞株動物氣管細(xì)胞動物腎細(xì)胞動物巨噬細(xì)胞人正常細(xì)胞人腫瘤細(xì)胞、癌細(xì)胞小鼠正常細(xì)胞小鼠腫瘤細(xì)胞、癌細(xì)胞大鼠正常細(xì)胞大鼠腫瘤細(xì)胞其它動物細(xì)胞查看全部產(chǎn)品

相關(guān)文章

詳細(xì)介紹

CRL-2586 EOMA 小鼠血管內(nèi)皮瘤細(xì)胞 的詳細(xì)介紹

ATCC^® Number:

CRL-2586™

Price:

$329.00

Designations:	EOMA
Depositors:	R Auerbach
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Mus musculus （mouse）
Morphology:	endothelial
Source:	Disease: hemangioendothelioma Strain: 129 Cell Type: endothelial
Cellular Products:	angiotensin converting enzyme （ACE） [51514] thrombospondin [51514] cathepsin L [47148] endostatin [47148] interleukin-6 （interleukin 6, IL-6） [53484]
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2586 EOMA 小鼠血管內(nèi)皮瘤細(xì)胞
Isolation:	Isolation date: 1980
Receptors:	acetylated low density liproprotein [51514]
Tumorigenic:	Yes
Antigen Expression:	CD31 + vascular addressin + CD45 （Ly5-T200） +
Age:	adult
Comments:	The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse. [51514] The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. [51514] Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. [47148] Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin （LEH）. [53484] The cells constitutively express the vascular addressin identified by antibody MECA-99. EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. [51514]
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. CRL-2586 *Subction Ratio:** A subction ratio of 1:3 to 1:6 is recommended Medium Renewal:* Every 2 to 3 days
Preservation:	Freeze medium: Complete growth medium supplemented with 5% DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 recommended serum:ATCC 30-2020 derivative:ATCC CRL-2587
References:	47148: Felbor U, et al. Secreted cathepsin L generates endostatin from collagen XVIII. EMBO J. 19: 1187-1194, 2000. PubMed: 10716919 51514: Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185 53484: Zhu XL, et al. Kinetics of cytokine gene expression in macrophage and endothelial cell lines following liposome encapsulated haemoglobin （LEH） treatment in vitro. Cytokine 8: 541-547, 1996. PubMed: 8891435 53486: Wen W, et al. The generation of endostatin is mediated by elastase.. Cancer Res. 59: 6052-6056, 1999. PubMed: 10626789

ATCC® Number: CRL-2586™ Price: $329.00

Designations: EOMA

Depositors: R Auerbach

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus （mouse）

Morphology: endothelial

CRL-2586

Source: Disease: hemangioendothelioma

Strain: 129

Cell Type: endothelial

Cellular Products: angiotensin converting enzyme （ACE） [51514]

thrombospondin [51514]

cathepsin L [47148]

endostatin [47148]

interleukin-6 （interleukin 6, IL-6） [53484]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1980

Receptors: acetylated low density liproprotein [51514]

Tumorigenic: Yes

Antigen Expression: CD31 +

vascular addressin +

CD45 （Ly5-T200） +

Age: adult

Comments: The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse. [51514]

The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. [51514]

Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. [47148]

Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin （LEH）. [53484]

The cells constitutively express the vascular addressin identified by antibody MECA-99.

EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. [51514]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

CRL-2586

產(chǎn)品咨詢

留言框

產(chǎn)品：
您的單位：
您的姓名：
聯(lián)系電話：
常用郵箱：
省份：
詳細(xì)地址：
補(bǔ)充說明：
驗證碼：

請輸入計算結(jié)果（填寫阿拉伯?dāng)?shù)字），如：三加四=7

上一篇：Y2 小鼠成纖維細(xì)胞
下一篇：CRL-6323B16F1 小鼠黑色素瘤細(xì)胞

美女扒开奶罩露出奶头视频网站|精品久久久久久中文字幕大豆网|男人边吃奶边做呻吟免费视频|一二三四免费观看在线视频中文版

EOMA 小鼠血管內(nèi)皮瘤細(xì)胞

留言框

產(chǎn)品：

您的單位：

您的姓名：

聯(lián)系電話：

常用郵箱：

省份：

詳細(xì)地址：

補(bǔ)充說明：

驗證碼：