美女扒开奶罩露出奶头视频网站|精品久久久久久中文字幕大豆网|男人边吃奶边做呻吟免费视频|一二三四免费观看在线视频中文版

咨詢熱線

15000266580

當前位置:首頁 >產品中心>菌種庫>酵母>亞膜漢遜酵母

亞膜漢遜酵母

簡要描述:亞膜漢遜酵母,感謝您使用本單位的微生物菌種,我們有可用于科研、教學、生產的菌種約2500株,基本覆蓋了環(huán)境、工業(yè)、農業(yè)、分析、食、藥用真菌行業(yè)各類生產和科研用微生物,其中許多優(yōu)良菌株的生產性能具有較高水平。

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2906

產品分類

Product Category

詳細介紹

亞膜漢遜酵母


注意事項

1、 微生物菌種應保藏于低溫、清潔和干燥的地方,室溫放置時間過長會導致菌種衰退;

2、 菌種操作應在無菌條件下進行,防止雜菌污染;

3、 斜面菌種保藏時間通常為3-6個月,應根據(jù)菌種狀況及時轉接;凍干菌種保藏時間通常為2-15年;


亞膜漢遜酵母

ATCC® Number:  CL-188™

Designations:  LS 174T

Depositors:   Northwestern University

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: colon

Tumor Stage: Dukes' type B

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA) (The production of CEA in the ATCC seed stock was 1944 ng per 10(6) cells in 10 days.)

interleukin 10 (IL-10) [22511]

interleukin 6 (IL-6)

mucin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

Reverse Transcript: negative

Oncogene: myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -

Antigen Expression: serologically defined colon cancer antigen 3; Homo sapiens, expressed

HLA A2, B13, B50; Blood type O

Cytogenetic Analysis: 45,X; one X chromosome missing; no other chromosomal aberrations

Isoenzymes:  ADA, 1

ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Age:  58 years

Gender:  female

Ethnicity:  Caucasian

Comments: LS 174T is a variant of LS 180 (ATCC CL-187) that has been maintained by using trypsin in the subculture protocol. It is more easily subc*ted than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA).

Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles [Pubmed ID: 1262041].

They are negative for p53 antigen expression, but positive for mRNA expression.

LS 174T cells stain positively for cytokeratins.

The line is positive for expression of c-myc, N-myc, H-ras, N-ras.

Myb, and fos oncogenes.

K-ras and sis oncogene expression were not detected.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derived from same individual:ATCC CL-187

References: 2148: Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041

3524: Tom BH, et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980

22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

22511: Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

32265: Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066

32377: Shibata D, et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat. Genet. 6: 273-281, 1994. PubMed: 8012390

32794: Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486



















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網(wǎng)    管理登陸
国产真实乱对白精彩久久老熟妇女 | 亚洲AⅤ永久无码精品AA| 欧美人与禽zozzo性伦交| 女人与公拘交酡全过程| 亚洲少妇色图在线观看| 亚洲精品熟女国产| 亚洲精品无码久久久久久久| 手机福利视频| 乌克兰少妇videos高潮| 亚洲av中文无码乱人伦| 国产精品久久久久乳精品爆| 成人在线男人天堂av| 成人免费av片在线观看| 亚洲av综合永久无码精品天堂 | 欧美丰满熟妇xx猛交| 人妻熟妇精品免费观看| 欧美成年黄网站色视频| 国产精品成人自拍视频| 亚洲国产精品一区二区久久| 加勒比精品视频在线观看| 欧美亚洲一区二区三区| 开心激情站开心激情网六月婷婷| 无码国产精品一区二区免费式直播| 日本高清va在线播放| 精品深夜av无码一区二区老年| 精品毛片乱码1区2区3区| 国内成+人 亚洲+欧美+综合在线 | 永久免费av无码网站国产| 国产顶级熟妇高潮xxxxx| 午夜不卡av免费| 亚洲一区二区三区av无码| 国产 在线 | 日韩| 女人下边被添全过视频的网址| 香蕉伊蕉伊中文视频在线| 中国熟妇浓毛hdsex| 北条麻妃一区二区三区av高清 | 麻豆妓女爽爽一区二区三| 国产亚洲av人片在线观看| 亚洲精品国产av天美传媒| 亚洲啪啪综合av一区| 国产情侣真实露脸在线|