亚洲日本精品国产第一区二区_香港经典a毛片免费观看hd_欧美放荡的少妇_亚洲va久久久噜噜噜久久_少妇装睡让我滑了进去

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>Panc 03.27 人胰腺癌細(xì)胞

Panc 03.27 人胰腺癌細(xì)胞

簡(jiǎn)要描述:CRL-2549 Panc 03.27
人胰腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
Panc 03.27 人胰腺癌細(xì)胞

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:1777

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

ATCC® Number: CRL-2549™ Price: $417.00

Designations: Panc 03.27

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

Tumorigenic: Yes

Oncogene: K-ras + (wild-type)

Antigen Expression: MHC class I +; MHC class II - [50655]

Blood type A; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 8,13

THO1: 6

TPOX: 8,9

vWA: 16,17

Age: 65 years

Gender: female

Ethnicity: White

Comments: Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 37.5 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602


CRL-2549





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品福利一区二区久久| 日韩av国内av一区二区| 国产自偷亚洲精品页65页| 色五月婷婷成人网| 中字幕一区二区三区乱码| 婷婷五月日韩av永久免费| 四虎国产精品免费久久| 人妻 日韩 欧美 综合 制服| 日韩精品成人无码专区免费| 亚洲 欧洲 日韩 综合二区| 奇米综合四色77777久久| 2021久久精品国产99国产精品| 国产乡下三级全黄三级bd| 亚洲国产av无码精品无广告| 亚洲性无码av在线欣赏网| 东北女人啪啪对白| 国产乱妇无乱码大黄aa片| 国产精品99久久久久久www| 欧美日韩亚洲tv不卡久久| 无码人妻丰满熟妇区五十路在线| 国产一区二区三区在线观看免费| 天堂8在线天堂资源bt| 国产裸拍裸体视频在线观看| 国产真实伦在线观看| 艳妇臀荡乳欲伦交换在线播放| 少妇裸交aa大片| 一区二区三区av天堂| 久久96国产精品久久久| 天天综合亚洲色在线精品| 老司机午夜福利视频| 自拍自产精品免费在线| 久久久久亚洲av成人片乱码| 狂野欧美激情性xxxx| 人妻少妇精品视频专区| 亚洲av成人一区二区三区| 国产熟妇勾子乱视频| 久热中文字幕在线精品观| 久久精品国产一区二区无码| 久久日韩乱码一二三四区别 | 爽爽影院免费观看| 亚洲乱色熟女一区二区|