亚洲日本精品国产第一区二区_香港经典a毛片免费观看hd_欧美放荡的少妇_亚洲va久久久噜噜噜久久_少妇装睡让我滑了进去

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-37人結(jié)腸腺癌細(xì)胞

人結(jié)腸腺癌細(xì)胞

簡(jiǎn)要描述:HTB-37 Caco-2 人結(jié)腸腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-37
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1954

產(chǎn)品分類

Product Category

詳細(xì)介紹

HTB-37 Caco-2 人結(jié)腸腺癌細(xì)胞

ATCC® Number: HTB-37™

Designations: Caco-2

Depositors: J Fogh

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

HTB-37 Caco-2 人結(jié)腸腺癌細(xì)胞

Source: Organ: colon

Disease: colorectal adenocarcinoma

Cellular Products: keratin

retinoic acid binding protein 1

retinol binding protein 2

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Receptors: heat stable enterotoxin (Sta, E. coli), expressed

epidermal growth factor (EGF), expressed

Virus Susceptibility: Human immunodeficiency virus 1

Tumorigenic: Yes

Reverse Transcript: N

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11,13,14

D16S539: 12,13

D5S818: 12,13

D7S820: 11,12

TH01: 6

TPOX: 9,11

vWA: 16,18

Cytogenetic Analysis: The stemline modal chromosome number is 96, occurring at 16% with polyploidy at 3.2%. Ten common markers were detected i.e., t(1q;?), 10q-, t(11q17q) and 7 others. The t(1q17q) and M11 were found in a portion of cells. The ins(2), 10q-, and t(15q;?) were generally paired, and t(11q;17q) and t(21q;?) were mostly three-copied. Normal N9 was absent, and N21 was lost in some cells. One to 4 small acrocentric chromosomes were detected. No Y chromosome with bright distal q-band was detected by Q-observation.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 1

PGM3, 1

Age: 72 years adult

Gender: male

Ethnicity: Caucasian

HeLa Markers: N

Comments: Upon reaching confluence, the cells express characteristics of enterocytic differentiation [PubMed ID: 1939345]. Caco-2 cells express retinoic acid binding protein I and retinol binding protein II [PubMed ID: 9040537].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.The recommended inoculum is 1 X 10(4) viable cells/cm2. Subculture cells when they are about 80% confluent, at a cell concentration between 8 X 10(4) and 1 X 10(5) cell/cm2.

Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended

Medium Renewal: 1 to 2 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Doubling Time: about 62 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derivative:ATCC CRL-2102

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

22409: Jumarie C, Malo C. Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J. Cell. Physiol. 149: 24-33, 1991. PubMed: 1939345

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22564: Adachi A, et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

23009: Cohen MB, et al. Receptors for Escherichia coli heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell. Physiol. 156: 138-144, 1993. PubMed: 8100232

HTB-37 Caco-2



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
小雪尝禁果又粗又大的视频| 日本一区二区三区内射| 免费观看啪啪黄的网站| 中国凸偷窥xxxx自由视频| 日韩中文字幕一区在线| aa级女人大片免费视频| 美女裸免费观看网站| 精品女同一区二区| 亚洲av无码码潮喷在线观看| 无码国产精品一区二区免费式芒果| 精品国产乱码久久久久久浪潮| 久久狼人国产综合精品| 99噜噜噜在线播放| 国产女同疯狂作爱系列| 久久精品女人天堂av麻| 18禁止午夜福利体验区| 97久久精品午夜一区二区| 亚洲熟妇av一区二区三区浪潮 | 国产公妇仑乱在线观看| 久久99国产乱子伦精品免费| 性高湖久久久久久久久| 人妻av无码专区| 四虎成人精品在永久免费| 曰本女人牲交全视频免费播放| 国精产品999一区二区三区有限| 亚洲av无码精品色午夜app| 色综合久久网| 亚洲精品国产精品乱码在线观看| 又黄又爽又猛的视频免费| 116少妇做爰毛片| 精品人妻无码区二区三区| 无码专区人妻系列日韩精品| 满春阁精品a∨在线观看| 特级aaaaaaaaa毛片免费视频| 最近中文字幕mv在线视频看| 特级a欧美做爰片第一次| 午夜福利免费一区二区| 6080yyy午夜理论片中无码| 亚洲国产精品午夜福利| 国产精品无码天天爽视频| 成人欧美一区二区三区在线|